recombinant proteins cxcl12 medchemexpress  (MedChemExpress)

Journal: NPJ Biofilms and Microbiomes

Article Title: Natural killer cell effector function is critical for host defense against alcohol-associated bacterial pneumonia

doi: 10.1038/s41522-024-00558-w

Figure Lengend Snippet: Mouse primary NK cells were collected from alcohol-fed and treated mice and added to the apical side of a Transwell with migration assessed in response to ( A ) media alone, and ( B ) media with CCL2/CXCL12 following 5 h of incubation. C Primary NK cells were harvested from control and binge-on-chronic alcohol-fed mice with and without treatment. NK cells were then incubated with K. pneumoniae for 3 h. and bacterial viability was determined by serial dilution. p -values as determined by one‐way ANOVA with Sidak’s multiple comparisons are shown in the figure. N = 3 wells of primary NK cells/group (6 mice/group, NK cells per 2 mice were pooled).

Article Snippet: Cat. No. 45-SRP3215-10UG), 100 ng/mL of CXCL12 (R&D Systems, Cat. No. 460-SD-050), 100 ng/mL of CXCL9 (R&D Systems, Cat. No. 492-MM-010), 100 ng/mL CXCL10 (R&D Systems, Cat. No. 466-CR-010), 100 ng/mL CXCL11 (R&D Systems, Cat. No. 572-MC-025), 300 ng/mL CX3CL1 (R&D Systems, Cat. No. 472-FF-025) was added to the bottom of the of the Transwell.

Techniques: Migration, Incubation, Control, Serial Dilution

Journal: NPJ Biofilms and Microbiomes

Article Title: Natural killer cell effector function is critical for host defense against alcohol-associated bacterial pneumonia

doi: 10.1038/s41522-024-00558-w

Figure Lengend Snippet: Mouse primary splenic NK cells were collected from alcohol-fed and treated mice and added to the apical side of a Transwell and migration in response to different chemokines was assessed following 5 h of incubation. A Confirmation of NK cell purification. (a1) Percentage of NK cells pre-isolation, and (a2) percentage of NK cells post-isolation. NK cell migration response profiles to ( B ) medial alone, ( C ) CCL2, ( D ) CXCL12, ( E ) CX3CL1, and ( F ) CCL9-11. Percent migration was calculated as the total number of viable NK cells in the bottom well divided by the total number of viable NK cells added to the Transwell insert. p values are indicated in the figure by one‐way ANOVA with Sidak’s multiple comparison. N = 6–7 wells of primary NK cells/group (12–14 mice/group, NK cells per 2 mice were pooled) derived from 2 independent experiments.

Article Snippet: Cat. No. 45-SRP3215-10UG), 100 ng/mL of CXCL12 (R&D Systems, Cat. No. 460-SD-050), 100 ng/mL of CXCL9 (R&D Systems, Cat. No. 492-MM-010), 100 ng/mL CXCL10 (R&D Systems, Cat. No. 466-CR-010), 100 ng/mL CXCL11 (R&D Systems, Cat. No. 572-MC-025), 300 ng/mL CX3CL1 (R&D Systems, Cat. No. 472-FF-025) was added to the bottom of the of the Transwell.

Techniques: Migration, Incubation, Purification, Isolation, Comparison, Derivative Assay

Journal: NPJ Biofilms and Microbiomes

Article Title: Natural killer cell effector function is critical for host defense against alcohol-associated bacterial pneumonia

doi: 10.1038/s41522-024-00558-w

Figure Lengend Snippet: Human NK cells (NK-92 cell line) were treated with alcohol, indole, or recombinant TGF-β1, and NK cell migration in response to the CCL2/CXCL12 was assessed. p values are indicated in the figure by one‐way ANOVA with Sidak’s multiple comparison. N = 9 wells of NK-92 NK cells/group derived from 3 independent experiments.

Article Snippet: Cat. No. 45-SRP3215-10UG), 100 ng/mL of CXCL12 (R&D Systems, Cat. No. 460-SD-050), 100 ng/mL of CXCL9 (R&D Systems, Cat. No. 492-MM-010), 100 ng/mL CXCL10 (R&D Systems, Cat. No. 466-CR-010), 100 ng/mL CXCL11 (R&D Systems, Cat. No. 572-MC-025), 300 ng/mL CX3CL1 (R&D Systems, Cat. No. 472-FF-025) was added to the bottom of the of the Transwell.

Techniques: Recombinant, Migration, Comparison, Derivative Assay

Journal: Cell reports

Article Title: Clostridioides difficile toxin B subverts germinal center and antibody recall responses by stimulating a drug-treatable CXCR4-dependent mechanism

doi: 10.1016/j.celrep.2024.114245

Figure Lengend Snippet: (A) Female B6 mice ( n = 4 per group) were given 1 ng TcdB2, 1 ng D270N, or PBS vehicle control by the s.c. route. After 48 h, splenocytes, B cells, or CD4 + T cells were isolated (B and CD4 + T cells by magnetic separation) and seeded into the top of a Transwell. The bottom of the Transwell contained serum-free medium with or without CXCR12. Cells were incubated for 6 h, and then migratory cells were fixed and stained with crystal violet and counted. (B) Quantification of migratory splenocytes averaged from 4 fields of view from each Transwell membrane (mean ± SD, n = 4 per group). Data are representative of two independent experiments. (C) Representative flow cytometry plots for isolated B cells and CD4 + T cells. Graphs depict quantification of migratory B cells and CD4 + T cells (mean ± SD, n = 4 per group). Data are representative of two independent experiments. (D) Isolated B cells from vehicle-, D270N-, and TcdB2-treated mice were stimulated in vitro with ligands for CXCR4, CXCR5, and CCR7 (CXCL12, CCL19/CCL21, and CXCL13, respectively). Data are pooled from two independent experiments (mean ± SD, n = 8 per group). (E) Isolated splenic B cells were cultured with vehicle, D270N, or TcdB2 for 6 h ( n = 3). Graph shows mean ± SD, and data are representative of 2 similar experiments. Statistical significance was determined by one-way ANOVA: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Mouse CXCL12 recombinant chemokine , Sino Biological , Cat # 50025-MNAE.

Techniques: Isolation, Incubation, Staining, Membrane, Flow Cytometry, In Vitro, Cell Culture

Journal: Cell reports

Article Title: Clostridioides difficile toxin B subverts germinal center and antibody recall responses by stimulating a drug-treatable CXCR4-dependent mechanism

doi: 10.1016/j.celrep.2024.114245

Figure Lengend Snippet: (A) Female B6 mice were given 1 ng TcdB2 or PBS vehicle control (i.p.) and then given either PBS vehicle or AMD3100 (1 or 10 μg/g of body weight) by the s.c. route. After 48 h, splenocytes were isolated, and migration toward CXCL12 was measured as described in . The graph depicts mean ± SD numbers of migratory splenocytes averaged from 4 fields of view from each Transwell membrane. Data are from 2 pooled experiments ( n = 7 mice per group). Statistical significance was determined by one-way ANOVA with Dunnett’s multiple-comparison post-test; **** p < 0.0001. (B) Mice ( n = 7 per group from 2 pooled experiments) were given 1 ng TcdB2 or PBS vehicle control (i.p.) and then immunized s.c. with 10 μg of B2Δ/Alum after 5 h. At 0, 24, and 48 h post PBS or TcdB2 treatment, mice were treated with AMD3100 or PBS vehicle control (i.p.). Graphs depict the mean ± SD area and number of GCs in iLNs collected 21 days post treatment. Statistical significance was determined by two-tailed t test. Images show representative H&E sections from lymph nodes. Yellow arrows indicate GCs. Thin dark lines were due to a crease in the section. The scale bar depicts 500 μm. (C) Mice were treated with PBS vehicle and then immunized with B2Δ/alum (B2Δ), treated with TcdB2 and then immunized (TcdB2 + B2Δ), immunized and then treated with AMD3100 (AMD + B2Δ), or treated with TcdB2, immunized, and treated with AMD3100 (AMD + TcdB2 + B2Δ). Sera were collected on day 60 (pre-boost), and a booster vaccine was administered before collection of sera on day 74 (post-boost). Data show mean titers ± SD for 5 mice per group. Significance was determined by two-way ANOVA with Sidak’s multiple comparison post-test. * p < 0.05.

Article Snippet: Mouse CXCL12 recombinant chemokine , Sino Biological , Cat # 50025-MNAE.

Techniques: Isolation, Migration, Membrane, Comparison, Two Tailed Test

Journal: Cell reports

Article Title: Clostridioides difficile toxin B subverts germinal center and antibody recall responses by stimulating a drug-treatable CXCR4-dependent mechanism

doi: 10.1016/j.celrep.2024.114245

Figure Lengend Snippet:

Article Snippet: Mouse CXCL12 recombinant chemokine , Sino Biological , Cat # 50025-MNAE.

Techniques: Virus, Recombinant, Expressing, Suspension, Protease Inhibitor, SYBR Green Assay, Cell Isolation, Binding Assay, Bradford Protein Assay, Cell Counting, Enzyme-linked Immunosorbent Assay, Software, Western Blot, Protein Extraction